Coding

Part:BBa_K364317

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-10-02)

NHR-31 LBD

Nuclear hormone receptor family member nhr-31.

The nhr-31 encodes one of over 200 C. elegans nuclear receptors. The nhr-31 activity is required for proper growth, development, and function of the excretory cell. In regulating excretory cell development, NHR-31 appears to function by controlling the expression of genes encoding subunits of the vacuolar ATPase. An nhr-31::gfp promoter fusion is expressed at high levels in the excretory cell beginning at embryogenesis and continuing through adulthood, with lower levels of expression seen in the intestine and unidentified tail cells.

Picture of gel electrophoresis: NHR-31 LBD in pSB1C3 resulting an insert of 597 bp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 229
    Illegal SapI.rc site found at 99


[edit]
Categories
Parameters
n/aNHR-31 LBD